Biotechnology approach for precision breeding of cold tolerance rice. - Link (March 2011)
Zhoa, X Dolferus, R
This RIRDC report present the research results undertaken with the focus on the development of technologies for enhancing breeding approaches for cold tolerant rice varieties. The report is aimed at the contributing organisations, Rural Industries Research and Development Corporation, the University of Sydney and CSIRO Plant Industry, the rice breeders, the scientific community involved in improving cold tolerance in rice and the rice growers and the Australian rice industry in general. There were 4 key aims including developing microspore culture as a tool for the generation of DH lines and DH populations.The preparation of mapping populations for 7 crosses and phenotyping of these populations for cold-tolerance under controlled environment conditions. QTL mapping of RIL populations of Doongara crossed to Lijiangheigu and Banjiemang. Finally identification of the molecular basis of cold-tolerance and its genetic basis. The method included 5 cold tolerant varieties in genetic crosses to the Australian cold-sensitive variety Reiziq. Please refer to method section for full method. The results of the project included microspore culture technology being developed although shows a lot of varietal differences. However, we have been able to successfully produce DH plants for all crosses involving the receptor variety for cold-tolerance, Reiziq. Seven RIL populations were developed using the cold-tolerant germplasm that was identified by the breeders as suitable for incorporation in breeding programs for cold tolerance. Two FRIL populations of Doongara × Lijiangheigu and Doongara × Banjiemang were used for cold tolerance QTL mapping using DArT and microsatellite marker techniques. Significant progress was made in understanding the molecular basis of pollen abortion and the tolerance mechanism that avoids pollen abortion in cold-tolerant germplasm. Despite the funding cuts during the life of this project we have been able to develop a set of tools that will be useful for future cold-tolerance breeding.