Construction of user friendly plant expression vectors using rice promoters. (2004)

Eamens, A

2004

This CRC project was undertaken to assist in developing user friendly plant expression vectors based on rice promoters. A PhD student Andrew Eamens had identified a reporter gene genes containing a minimal promoter (enhancer trap) or intron splice acceptors (gene trap) in T-DNA or transposon tagging systems, several promoter sequences and these were used to produce plant expression vectors with tissue specific expression. Andrew was employed fro three months to continue the work and during this time was able to construct a small group of user friendly plat expression vectors with tissue specific expression promoters by using double right boarder (DRB) that had been previously developed. During this project a new base binary vector construct (PDRB12dn) was constructed. The project also saw a total of 12 promoter fragments were also amplified by the polymerase chain reaction (PCR), ready for addition to the base vector. The project began the progress of cloning of individual promoter fragments. This project and its results will allow plant expression constructs being produced to enable the production of selectable marker free transgenic plants expressing GOIs in specific cells, tissues or organs.